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Thermo Fisher avidind (1∶400, texas red–conjugated avidin
Oxidative stressed cells were detected using <t>AvidinD</t> (red) as a marker (DAPI, blue). In the control group (A–C) as well as in the NP-exposed groups the vast majority of AvidinD-positive cells were located within the ONL. AgNO 3 - treated groups displayed abundant numbers of Avidin-positive cells. Graph shows numbers of AvidinD-positive cells and results are presented as mean ±SD (n = 4 explants/group). * p <0.05 compared to control. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Scale bars equal 100 µm.
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Oxidative stressed cells were detected using AvidinD (red) as a marker (DAPI, blue). In the control group (A–C) as well as in the NP-exposed groups the vast majority of AvidinD-positive cells were located within the ONL. AgNO 3 - treated groups displayed abundant numbers of Avidin-positive cells. Graph shows numbers of AvidinD-positive cells and results are presented as mean ±SD (n = 4 explants/group). * p <0.05 compared to control. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Scale bars equal 100 µm.

Journal: PLoS ONE

Article Title: Silver and Gold Nanoparticles Exposure to In Vitro Cultured Retina – Studies on Nanoparticle Internalization, Apoptosis, Oxidative Stress, Glial- and Microglial Activity

doi: 10.1371/journal.pone.0105359

Figure Lengend Snippet: Oxidative stressed cells were detected using AvidinD (red) as a marker (DAPI, blue). In the control group (A–C) as well as in the NP-exposed groups the vast majority of AvidinD-positive cells were located within the ONL. AgNO 3 - treated groups displayed abundant numbers of Avidin-positive cells. Graph shows numbers of AvidinD-positive cells and results are presented as mean ±SD (n = 4 explants/group). * p <0.05 compared to control. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Scale bars equal 100 µm.

Article Snippet: Six sections per specimen (together representing the whole specimen) were stained with AvidinD (1∶400, Texas Red–conjugated avidin; Molecular Probes Inc., Eugene, OR, USA; A-820).

Techniques: Marker, Avidin-Biotin Assay